Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
MilliporeSigma™ Calbiochem™ OmniPur™ TBE Buffer, 10X
Suitable for a wide range of molecular biology applications. Each liter of 10X TBE buffer yields 10 liters of 1X TBE buffer.
| Physical Form | Liquid |
|---|
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.5
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
ACES, Biological Buffer, 98%, Spectrum™ Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
MilliporeSigma™ Tris Hydrochloride, OmniPur™, Calbiochem™,
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride; 1,3-Propanediol, 2-amino-2- (hydroxymethyl)-, hydrochloride (1:1); 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane, hydrochloride; Tris hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| Synonym | 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride; 1,3-Propanediol, 2-amino-2- (hydroxymethyl)-, hydrochloride (1:1); 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane, hydrochloride; Tris hydrochloride |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
ADA, 0.2M buffer soln., pH 6.0, Thermo Scientific™
CAS: 7415-22-7 Molecular Formula: C6H9N2NaO5 Molecular Weight (g/mol): 212.137 MDL Number: MFCD00065483 InChI Key: QCGKKVWCABJQQI-UHFFFAOYSA-M PubChem CID: 23679048 IUPAC Name: sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate SMILES: C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]
| PubChem CID | 23679048 |
|---|---|
| CAS | 7415-22-7 |
| Molecular Weight (g/mol) | 212.137 |
| MDL Number | MFCD00065483 |
| SMILES | C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+] |
| IUPAC Name | sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate |
| InChI Key | QCGKKVWCABJQQI-UHFFFAOYSA-M |
| Molecular Formula | C6H9N2NaO5 |
Thermo Scientific Chemicals HEPES, 0.5M buffer soln., pH 8.5
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
MilliporeSigma™ TBE Buffer, 10X, Molecular Biology Grade, Calbiochem™,
A 10X concentrate that can be diluted to a 1X solution containing 89mM Tris, 89mM boric acid, 2mM EDTA
Thermo Scientific Chemicals MOPS-SDS running buffer (20X)
MOPS-SDS running buffer (20X) is a buffer frequently used for separating medium- to large-sized proteins on a gel.
| Health Hazard 3 | P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P362-P501c |
|---|---|
| Health Hazard 2 | GHS H Statement H315-H319-H335 Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation. |
| Solubility Information | It is soluble in water. |
| Physical Form | Liquid |
| Chemical Name or Material | MOPS-SDS running buffer |
| TSCA | No |
| Recommended Storage | Ambient temperatures |
| Concentration | 20X |
ACES, 0.5M buffer soln., pH 6.5, Thermo Scientific™
CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N
| PubChem CID | 81832 |
|---|---|
| CAS | 7365-82-4 |
| Molecular Weight (g/mol) | 182.194 |
| ChEBI | CHEBI:39062 |
| MDL Number | MFCD00008030 |
| SMILES | C(CS(=O)(=O)O)NCC(=O)N |
| IUPAC Name | 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid |
| InChI Key | DBXNUXBLKRLWFA-UHFFFAOYSA-N |
| Molecular Formula | C4H10N2O4S |
Thermo Scientific Chemicals MOPS, 1.0M buffer soln., pH 7.0
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| IUPAC Name | 3-morpholin-4-ylpropane-1-sulfonic acid |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
MP Biomedicals™ Dulbecco's Phosphate Buffered Saline (10X) without Calcium and Magnesium
Stock solution used to prepare 1X D-PBS in cell culture grade water.